Plants can crash when photosynthesis rates are high

  • Jul 17, 2019
  • plant growth, photosynthesis, Faculty, Research
  • Homepage News, Faculty & Staff, Research, Biochemistry, College of Natural Science, Plant Research Laboratory
Image of Calvin-Benson cycle
When photosynthetic activity is high, genes responsible for starch synthesis get turned on rapidly (red boxes) while genes in almost every other aspect of carbon dioxide metabolism are turned down (dark blue boxes). GPT2 and GAPN are highlighted (light blue boxes). Graphic by Sean Weise.

Just as brakes are essential to whiz down a highway, plants rely on special proteins to maintain high rates of photosynthesis without crashing. In a new study, members of Michigan State University  Distinguished Professor Thomas Sharkey’s lab delve into a brake like protein, called GPT2. It helps manage photosynthesis in the presence of high levels of light or carbon dioxide, which can push photosynthesis into overdrive mode. 

The study was recently published in the journal, Frontiers in Plant Science.

The big picture is this. Photosynthesis is how plants make food for us all. Improving it is one of the last scientific barriers to increase crop yields and feed the world’s growing population. But researchers can’t simply force plants to put the pedal to the metal. That would be disastrous, because their brakes are more complex than a car’s.

GPT2 is part of the photosynthetic processes that introduce carbon into our diets. It is carbon that becomes the sugars and starches that fuel life.

“Starch is a plant’s backup battery,” said Sean Weise, research assistant professor in the Sharkey lab. “A plant builds it up during the day when it can do photosynthesis. It uses the starch at night when it can’t, because the sun is gone.”

GPT2 sits in the chloroplast membrane and helps manage that starch production by allowing sugars to move into the chloroplast. 

When photosynthesis increases rapidly, like when the sun peaks out from a cloudy day, GPT2 is quickly turned on. 

“We think this recycles sugars back into the chloroplast for starch production,” Sharkey said. “Other genes responsible for starch synthesis also get turned on rapidly. However, genes involved in almost every other aspect of carbon dioxide metabolism, including sucrose synthesis, are turned down. That last part was a surprise to us, and we’re looking into it.” 

The team thinks plants activate GPT2 as a sort of brake to allow the plant’s cells to keep up with increased photosynthetic activity and to increase starch stores for use at night.

When photosynthesis activity is high, a lot of energy compounds are pumped from the chloroplast into the rest of the plant cell. Most of the time, this is desired, but too many of these compounds can cause chemical reactions that harm the plant cell.

That’s one situation where plants step on the brakes.

“When GPT2 is active, another gene, called GAPN, that processes the high energy compounds that end up in the cytosol, is muzzled,” Weise said. “That is how plants recycle compounds back into the chloroplast to keep them away from the rest of the plant cell.”

Stepping back, Weise considers the big picture: improving photosynthesis.

“At first blush, GPT2 seems counterproductive. High rates of photosynthesis are good,” he said. “Why would a plant reduce its export of high energy compounds? Why put the brakes on productivity gains and instead recycle resources? However, when we look at plant metabolism more closely, we realize that there really can be too much of a good thing, and this could damage the plant cell."

“Understanding the brakes only gives us a more complete view of photosynthetic plant metabolism,” Sharkey added. “It also identifies new targets for improvement. Perhaps, in the future, we can engineer plants that don’t need to put on the brakes as often.”

In addition to Sharkey and Weise, MSU faculty members Sarathi M. Weraduwage (Department of Biochemistry), Jin Chen (MSU-DOE Plant Research Lab and Department of Computer Science and Engineering) and Fransisca C. Anozie (Department of Biochemistry and Molecular Biology), and Alejandro Morales, Centre for Crop Systems Analysis, Wageningen University, Wageningen, Netherlands, participated in the study. 

 

Banner Image: Photosynthesis is how plants make food for us all. Improving it is one of the last scientific barriers to increase crop yields and feed the world’s growing population. MSU courtesy photo.

 

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